DSSR-PyMOL cartoon-block schematics: PDB entry 2g1g

PDB-id

2g1g; DSSR-derived features in text and JSON formats

Class

RNA

Method

NMR

Summary

Solution structure of the anticodon loop of s. pombe trnai including the naturally occurring n6-threonyl adenine

Reference

Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P (2006): "The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif." Nucleic Acids Res., 34, 2878-2886. doi: 10.1093/nar/gkl081.

Abstract

Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.