Summary information and primary citation
- PDB-id
-
353d;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- X-ray (2.4 Å)
- Summary
- Crystal structure of domain a of thermus flavus 5s rrna
and the contribution of water molecules to its
structure
- Reference
-
Betzel C, Lorenz S, Furste JP, Bald R, Zhang M, Schneider
TR, Wilson KS, Erdmann VA (1994): "Crystal
structure of domain A of Thermus flavus 5S rRNA and the
contribution of water molecules to its structure."
FEBS Lett., 351, 159-164. doi:
10.1016/0014-5793(94)00834-5.
- Abstract
- This is the first high resolution crystal structure of
an RNA molecule made by solid phase chemical synthesis and
representing a natural RNA. The structure of the domain A
of Thermus flavus ribosomal 5S RNA is refined to R = 18% at
2.4 A including 159 solvent molecules. Most of the
2'-hydroxyl groups as well as the phosphate oxygens are
involved either in specific hydrogen bonds in
intermolecular contacts or to solvent molecules. The two
U-G and G-U base-pairs are stabilized by H-bonds supplied
via three water molecules to compensate for the lack of
base-pair hydrogen bonds. The structure shows for the first
time in detail the importance of highly ordered internal
water in stabilizing an RNA structure.
