Summary information and primary citation
- PDB-id
-
5eyq;
DSSR-derived features in text and
JSON formats
- Class
- DNA
- Method
- X-ray (2.3 Å)
- Summary
- Racemic crystal structures of pribnow box consensus
promoter sequence (pnna)
- Reference
-
Mandal PK, Collie GW, Srivastava SC, Kauffmann B, Huc I
(2016): "Structure
elucidation of the Pribnow box consensus promoter
sequence by racemic DNA crystallography." Nucleic
Acids Res., 44, 5936-5943. doi:
10.1093/nar/gkw367.
- Abstract
- It has previously been shown that the use of racemic
mixtures of naturally chiral macromolecules such as protein
and DNA can significantly aid the crystallogenesis process,
thereby addressing one of the major bottlenecks to
structure determination by X-ray crystallographic
methods-that of crystal growth. Although previous studies
have provided convincing evidence of the applicability of
the racemic crystallization technique to DNA through the
study of well-characterized DNA structures, we sought to
apply this method to a historically challenging DNA
sequence. For this purpose we chose a
non-self-complementary DNA duplex containing the
biologically-relevant Pribnow box consensus sequence
'TATAAT'. Four racemic crystal structures of this
previously un-crystallizable DNA target are reported (with
resolutions in the range of 1.65-2.3 Å), with further
crystallographic studies and structural analysis providing
insight into the racemic crystallization process as well as
structural details of this highly pertinent DNA
sequence.
