Summary information and primary citation
- PDB-id
-
6msf;
DSSR-derived features in text and
JSON formats
- Class
- virus-RNA
- Method
- X-ray (2.8 Å)
- Summary
- F6 aptamer ms2 coat protein complex
- Reference
-
Convery MA, Rowsell S, Stonehouse NJ, Ellington AD, Hirao
I, Murray JB, Peabody DS, Phillips SE, Stockley PG
(1998): "Crystal
structure of an RNA aptamer-protein complex at 2.8 A
resolution." Nat.Struct.Biol.,
5, 133-139. doi: 10.1038/nsb0298-133.
- Abstract
- The crystal structure, at 2.8 A resolution, of an RNA
aptamer bound to bacteriophage MS2 coat protein has been
determined. It provides an opportunity to compare the
interactions of MS2 coat protein and wild type operator
with those of an aptamer, whose secondary structure differs
from the wild type RNA in having a three-base loop
(compared to a tetraloop) and an additional base pair
between this loop and the sequence-specific recognition
element in the stem. The RNA binds in the same location on
the coat protein as the wild type operator and maintains
many of the same RNA-protein interactions. In order to
achieve this, the RNA stem loop undergoes a concerted
rearrangement of the 3' side while leaving the 5' side and
the loop interactions largely unchanged, illustrating the
ability of RNA to present similar molecular recognition
surfaces from distinct primary and secondary
structures.
