Summary information and primary citation
- PDB-id
-
7q2s;
DSSR-derived features in text and
JSON formats
- Class
- virus
- Method
- cryo-EM (3.7 Å)
- Summary
- Cryo idpc-stem structure recorded with csa 4.5
- Reference
-
Lazic I, Wirix M, Leidl ML, de Haas F, Mann D, Beckers M,
Pechnikova EV, Muller-Caspary K, Egoavil R, Bosch EGT,
Sachse C (2022): "Single-particle
cryo-EM structures from iDPC-STEM at near-atomic
resolution." Nat.Methods,
19, 1126-1136. doi: 10.1038/s41592-022-01586-0.
- Abstract
- In electron cryomicroscopy (cryo-EM), molecular images
of vitrified biological samples are obtained by
conventional transmission microscopy (CTEM) using large
underfocuses and subsequently computationally combined into
a high-resolution three-dimensional structure. Here, we
apply scanning transmission electron microscopy (STEM)
using the integrated differential phase contrast mode also
known as iDPC-STEM to two cryo-EM test specimens, keyhole
limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The
micrographs show complete contrast transfer to high
resolution and enable the cryo-EM structure determination
for KLH at 6.5 Å resolution, as well as for TMV at 3.5 Å
resolution using single-particle reconstruction methods,
which share identical features with maps obtained by CTEM
of a previously acquired same-sized TMV data set. These
data show that STEM imaging in general, and in particular
the iDPC-STEM approach, can be applied to vitrified
single-particle specimens to determine near-atomic
resolution cryo-EM structures of biological
macromolecules.
