Summary information and primary citation
- PDB-id
-
2l3c;
DSSR-derived features in text and
JSON formats
- Class
- hydrolase-RNA
- Method
- NMR
- Summary
- Solution structure of adar2 dsrbm1 bound to lsl
RNA
- Reference
-
Stefl R, Oberstrass FC, Hood JL, Jourdan M, Zimmermann M,
Skrisovska L, Maris C, Peng L, Hofr C, Emeson RB, Allain
FH (2010): "The
Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals
a Sequence-Specific Readout of the Minor Groove."
Cell(Cambridge,Mass.), 143,
225-237. doi: 10.1016/j.cell.2010.09.026.
- Abstract
- Sequence-dependent recognition of dsDNA-binding
proteins is well understood, yet sequence-specific
recognition of dsRNA by proteins remains largely unknown,
despite their importance in RNA maturation pathways.
Adenosine deaminases that act on RNA (ADARs) recode genomic
information by the site-selective deamination of adenosine.
Here, we report the solution structure of the ADAR2
double-stranded RNA-binding motifs (dsRBMs) bound to a
stem-loop pre-mRNA encoding the R/G editing site of GluR-2.
The structure provides a molecular basis for how dsRBMs
recognize the shape, and also more surprisingly, the
sequence of the dsRNA. The unexpected direct readout of the
RNA primary sequence by dsRBMs is achieved via the minor
groove of the dsRNA and this recognition is critical for
both editing and binding affinity at the R/G site of
GluR-2. More generally, our findings suggest a solution to
the sequence-specific paradox faced by many
dsRBM-containing proteins that are involved in
post-transcriptional regulation of gene expression.
