Summary information and primary citation
- PDB-id
-
2xdb;
DSSR-derived features in text and
JSON formats
- Class
- toxin-RNA
- Method
- X-ray (2.55 Å)
- Summary
- A processed non-coding RNA regulates a bacterial
antiviral system
- Reference
-
Blower TR, Pei XY, Short FL, Fineran PC, Humphreys DP,
Luisi BF, Salmond GPC (2011): "A
Processed Noncoding RNA Regulates an Altruistic Bacterial
Antiviral System." Nat.Struct.Mol.Biol.,
18, 185. doi: 10.1038/NSMB.1981.
- Abstract
- The ≥ 10³⁰ bacteriophages on Earth relentlessly drive
adaptive coevolution, forcing the generation of protective
mechanisms in their bacterial hosts. One such bacterial
phage-resistance system, ToxIN, consists of a protein toxin
(ToxN) that is inhibited in vivo by a specific RNA
antitoxin (ToxI); however, the mechanisms for this toxicity
and inhibition have not been defined. Here we present the
crystal structure of the ToxN-ToxI complex from
Pectobacterium atrosepticum, determined to 2.75-Å
resolution. ToxI is a 36-nucleotide noncoding RNA
pseudoknot, and three ToxI monomers bind to three ToxN
monomers to generate a trimeric ToxN-ToxI complex. Assembly
of this complex is mediated entirely through extensive
RNA-protein interactions. Furthermore, a 2'-3' cyclic
phosphate at the 3' end of ToxI, and catalytic residues,
identify ToxN as an endoRNase that processes ToxI from a
repetitive precursor but is regulated by its own catalytic
product.
